5 × m‐mlv reverse transcriptase buffer (Promega)

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Promega 5 × m‐mlv reverse transcriptase buffer
5 × M‐Mlv Reverse Transcriptase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+reverse-transcriptase+buffer/mgcl2/pmc11193114-93-10-14
Average 90 stars, based on 1 article reviews

5 × m‐mlv reverse transcriptase buffer - by Bioz Stars, 2026-09

90/100 stars

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Polymerase Chain Reaction:

Article Title: Empagliflozin inhibits increased Na influx in atrial cardiomyocytes of patients with HFpEF
Article Snippet: .. mRNA was extracted from right atrial biopsies using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendation. cDNA was transcribed from 1 μg RNA using random primers, PCR nucleotide mix, RNasin ribonuclease inhibitor, reverse transcriptase, and reverse transcriptase 5 × reaction buffer (Promega GmbH, Walldorf, Germany) for 1 h at 37°C. mRNA Abundance of Na V 1.5 ( SCN5A ; Assay ID: Hs00165693_m1 ) and β-actin ( ACTB ; Assay ID: Hs99999903_m1) was measured using the TaqMan Gene Expression (Thermo Fisher Scientific GmbH, Dreieich, Germany) detection method. ..

Reverse Transcription:

Gene Expression:

other:

Article Title: Expression of cell wall related genes in basal and ear internodes of silking brown-midrib-3 , caffeic acid O -methyltransferase (COMT) down-regulated, and normal maize plants
Article Snippet: Total RNA was isolated as described above. cDNA synthesis was conducted by incubating 1 μl of DNase-treated total RNA (1 μg/μl) with 2 μl of Oligo(dT)15 Primer (Promega, 500 μg/ml) and 15 μl of ultra-pure water for 5 min at 70°C and cooled down on ice for 5 min. 5 μl of Promega's MuMLVRT reverse transcriptase 5× buffer, 1.3 μl of 10 mM dNTP and 200 units of MuMLV-RT reverse transcriptase (Promega) were added to the denatured RNA and incubated at 37°C for 1 h. The reaction was stopped during 5 min at 70°C and then 5 min on ice.

Article Title: Polyglutamylation of microtubules drives neuronal remodeling
Article Snippet: 5 μl of M-MLV Reverse Transcriptase Buffer (Promega #M531A), 10 mM NTPs mixture, and 200 μ of MMLV (Promega #M170A) were added, and the mixture was incubated for 1.5 h at 37 °C. cDNA was purified with a QIAEX II Gel Extraction Kit (Qiagen #20021) as follows: The obtained cDNA was mixed with 2 μl QiaEX-II suspension (Silica-Matrix) and 80 μl QX-I buffer, incubated for 20 min at 25 °C at 1000 rpm and centrifuged at 13,000 x g for 2 min.

Article Title: MUC21 induces the viability and migration of glioblastoma via the STAT3/AKT pathway
Article Snippet: Total RNA was reverse transcribed into cDNA at 42 ̊C for 1 h using M-MLV reverse transcriptase (cat. no. M1701; Promega Corporation, includes M-MLV 5X Reaction Buffer 5 μl, dNTP, 10 mM 1.25 μl, Recombinant RNasin ® Ribonuclease Inhibitor 25 units, M-MLV RT 200 units and Nuclease-Free Water to final volume of 25 μl). qPCR was then performed using the SYBR Ex Taq kit (cat. no. 638319; Takara Bio, Inc.) and used according to the manufacturer's protocol.

Article Title: Polyglutamylation of microtubules drives neuronal remodeling
Article Snippet: 5 μl of M-MLV Reverse Transcriptase Buffer (Promega #M531A), 10 mM NTPs mixture and 200 U of MMLV (Promega #M170A) were added and the mixture incubated for 1.5 h at 37 °C. cDNA was purified with a QIAEX II Gel Extraction Kit (Qiagen #20021) as follows: The obtained cDNA was mixed with 2 μl QiaEX-II suspension (Silica-Matrix) and 80 μl QX-I buffer, incubated for 20 minutes at 25 °C at 1,000 rpm and centrifuged at 13,000 x g for 2 minutes.

Article Title: SNORD99 promotes endometrial cancer development by inhibiting GSDMD ‐mediated pyroptosis through 2'‐O‐methylation modification
Article Snippet: Subsequently, after the addition of 4 μL of 5 × M‐MLV Reverse Transcriptase buffer (PROMEGA), the mixture was subjected to incubation at 50°C for a duration of 1 h, 1 μL of 0.1 M DL‐Dithiothreitol (PROMEGA), and 1 μL of 40 u/μL RNase inhibitor (PROMEGA), followed by the incubation that was carried out at 70 C for a duration of 15 min.

Article Title: Rapid display method in translational synthesis of peptide
Article Snippet: Then, the washed magnetic beads were suspended in 10 μL of a reverse transcription reaction solution (5 units/μL M-MLV Reverse Transcriptase (Promega), 2 μM CGS3an21.R44, 0.5 mM dNTP, 10 mM Tris-HCl (pH 8.3), 15 mM potassium chloride, 0.6° C. mM magnesium chloride, 2 mM dithiothreitol), and the suspension was warmed at 42° C. for 1 hr to perform reverse transcription reaction.

Article Title: Hepatic hydrogen sulfide levels are reduced in mouse model of Hutchinson-Gilford progeria syndrome.
Article Snippet: Synthesis of cDNA was then performed by adding 10 uL master mix (1 uL Promega M-MLV reverse transcriptase, 5 uL Promega M-MLV 5× buffer, 5 uL pooled 10 mM dNTPs, and 0.625 uL RNAseOUT 40 units/uL) to the first stand sample and heating to 37°C for 1 hour.

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